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Key publications Breckpot Group

CS1-specific single-domain antibodies labeled with Actinium-225 prolong survival and increase CD8+ T cells and PD-L1 expression in Multiple Myeloma


Oncoimmunology. 2021 Nov 9;10(1):2000699. doi: 10.1080/2162402X.2021.2000699. eCollection 2021.


Multiple myeloma (MM) is a hematological malignancy characterized by the presence of clonal plasma cells in the bone marrow niche. Despite significant therapeutic advances, MM...

Unraveling the Effects of a Talimogene Laherparepvec (T-VEC)-Induced Tumor Oncolysate on Myeloid Dendritic Cells


Front Immunol. 2021 Oct 28;12:733506. doi: 10.3389/fimmu.2021.733506. eCollection 2021.


T-VEC, a HSV-1 derived oncolytic virus, is approved for the treatment of advanced melanoma. The mechanisms that underly the systemic anti-tumor effect that is seen following...

Plasma zinc status and hyperinflammatory syndrome in hospitalized COVID-19 patients: An observational study


Int Immunopharmacol. 2021 Nov;100:108163. doi: 10.1016/j.intimp.2021.108163. Epub 2021 Sep 20.


Zinc deficiency is associated with impaired antiviral response, cytokine releasing syndrome (CRS), and acute respiratory distress syndrome. Notably, similar complications are...

T-cell subsets in the skin and their role in inflammatory skin disorders


Allergy. 2021 Sep 24. doi: 10.1111/all.15104. Online ahead of print.


T lymphocytes (T cells) are major players of the adaptive immune response. Naive T cells are primed in the presence of cytokines, leading to polarization into distinct T-cell subsets with specific...

Formatting and gene-based delivery of a human PD-L1 single domain antibody for immune checkpoint blockade


Mol Ther Methods Clin Dev. 2021 Jun 4;22:172-182. doi: 10.1016/j.omtm.2021.05.017. eCollection 2021 Sep 10.


Monoclonal antibodies that target the inhibitory immune checkpoint axis consisting of programmed cell death protein 1 (PD-1) and its ligand, PD-L1, have changed...

TNFα and Immune Checkpoint Inhibition: Friend or Foe for Lung Cancer?


Int J Mol Sci. 2021 Aug 13;22(16):8691. doi: 10.3390/ijms22168691.


Tumor necrosis factor-alpha (TNFα) can bind two distinct receptors (TNFR1/2). The transmembrane form (tmTNFα) preferentially binds to TNFR2. Upon tmTNFα cleavage by the TNF-alpha-converting enzyme (TACE...

Functional Th1-oriented T follicular helper cells that infiltrate human breast cancer promote effective adaptive immunity


J Clin Invest. 2021 Oct 1;131(19):e139905. doi: 10.1172/JCI139905.


We previously demonstrated that tumor-infiltrating lymphocytes (TIL) in human breast cancer sometimes form organized tertiary lymphoid structures (TLS) characterized by CXCL13-producing T follicular...

Artificial Intelligence in PET: An Industry Perspective


PET Clin. 2021 Oct;16(4):483-492. doi: 10.1016/j.cpet.2021.06.006. Epub 2021 Aug 3.


Artificial intelligence (AI) has significant potential to positively impact and advance medical imaging, including positron emission tomography (PET) imaging applications. AI has the...

Measurement of hypoxia in the lung in idiopathic pulmonary fibrosis: an F-MISO PET/CT study


Eur Respir J. 2021 Oct 7;58(4):2004584. doi: 10.1183/13993003.04584-2020. Print 2021 Oct.


PMID: 34244317 | DOI: 10.1183/13993003.04584-2020

Motion estimation and correction for simultaneous PET/MR using SIRF and CIL


Philos Trans A Math Phys Eng Sci. 2021 Aug 23;379(2204):20200208. doi: 10.1098/rsta.2020.0208. Epub 2021 Jul 5.


SIRF is a powerful PET/MR image reconstruction research tool for processing data and developing new algorithms. In this research, new developments to SIRF are...

Synergistic tomographic image reconstruction: part 2


Philos Trans A Math Phys Eng Sci. 2021 Aug 23;379(2204):20210111. doi: 10.1098/rsta.2021.0111. Epub 2021 Jul 5.


This special issue is the second part of a themed issue that focuses on synergistic tomographic image reconstruction and includes a range of contributions in...

Site-Specific Radiolabeling of a Human PD-L1 Nanobody via Maleimide-Cysteine Chemistry


Pharmaceuticals (Basel). 2021 Jun 8;14(6):550. doi: 10.3390/ph14060550.


Immune checkpoint inhibitors targeting the programmed cell death-1 (PD-1) and its ligand PD-L1 have proven to be efficient cancer therapies in a subset of patients. From all the patients with various...

The antigen-binding moiety in the driver's seat of CARs


Med Res Rev. 2021 May 24. doi: 10.1002/med.21818. Online ahead of print.


Immuno-oncology has been at the forefront of cancer treatment in recent decades. In particular immune checkpoint and chimeric antigen receptor (CAR)-T cell therapy have achieved spectacular results...

Validation of a combined image derived input function and venous sampling approach for the quantification of [<sup>18</sup>F]GE-179 PET binding in the brain


Neuroimage. 2021 Aug 15;237:118194. doi: 10.1016/j.neuroimage.2021.118194. Epub 2021 May 21.


Blood-based kinetic analysis of PET data relies on an accurate estimate of the arterial plasma input function (PIF). An alternative to invasive measurements from arterial...

(An overview of) Synergistic reconstruction for multimodality/multichannel imaging methods


Philos Trans A Math Phys Eng Sci. 2021 Jun 28;379(2200):20200205. doi: 10.1098/rsta.2020.0205. Epub 2021 May 10.


Imaging is omnipresent in modern society with imaging devices based on a zoo of physical principles, probing a specimen across different wavelengths, energies...

Synergistic tomographic image reconstruction: part 1


Philos Trans A Math Phys Eng Sci. 2021 Jun 28;379(2200):20200189. doi: 10.1098/rsta.2020.0189. Epub 2021 May 10.


This special issue focuses on synergistic tomographic image reconstruction in a range of contributions in multiple disciplines and various application areas....

Imitation learning for improved 3D PET/MR attenuation correction


Med Image Anal. 2021 Jul;71:102079. doi: 10.1016/ Epub 2021 Apr 16.


The assessment of the quality of synthesised/pseudo Computed Tomography (pCT) images is commonly measured by an intensity-wise similarity between the ground truth CT and the pCT....

Overcoming the Challenges of High Quality RNA Extraction from Core Needle Biopsy


Biomolecules. 2021 Apr 22;11(5):621. doi: 10.3390/biom11050621.


The use of gene expression profiling (GEP) in cancer management is rising, as GEP can be used for disease classification and diagnosis, tailoring treatment to underlying genetic determinants of...

Fractionated Radiation Severely Reduces the Number of CD8+ T Cells and Mature Antigen Presenting Cells Within Lung Tumors


Int J Radiat Oncol Biol Phys. 2021 Sep 1;111(1):272-283. doi: 10.1016/j.ijrobp.2021.04.009. Epub 2021 Apr 15.


PURPOSE: The combination of standard-of-care radiation therapy (RT) with immunotherapy is moving to the mainstream of non-small cell lung cancer treatment....

Epigenetic Modifiers: Anti-Neoplastic Drugs With Immunomodulating Potential


Front Immunol. 2021 Mar 30;12:652160. doi: 10.3389/fimmu.2021.652160. eCollection 2021.


Cancer cells are under the surveillance of the host immune system. Nevertheless, a number of immunosuppressive mechanisms allow tumors to escape protective responses and impose immune...

Immunogenicity Risk Profile of Nanobodies


Front Immunol. 2021 Mar 9;12:632687. doi: 10.3389/fimmu.2021.632687. eCollection 2021.


Nanobodies (Nbs), the variable domains of camelid heavy chain-only antibodies, are a promising class of therapeutics or in vivo imaging reagents entering the clinic. They possess...

A multi-channel uncertainty-aware multi-resolution network for MR to CT synthesis


Appl Sci (Basel). 2021 Feb 12;11(4):1667. doi: 10.3390/app11041667. eCollection 2021 Feb.


Synthesising computed tomography (CT) images from magnetic resonance images (MRI) plays an important role in the field of medical image analysis, both for quantification and...

Single-Domain Antibody Nuclear Imaging Allows Noninvasive Quantification of LAG-3 Expression by Tumor-Infiltrating Leukocytes and Predicts Response of Immune Checkpoint Blockade


J Nucl Med. 2021 Nov;62(11):1638-1644. doi: 10.2967/jnumed.120.258871. Epub 2021 Mar 12.


Recent advances in the field of immune-oncology led to the discovery of next-generation immune checkpoints (ICPs). Lymphocyte activation gene-3 (LAG-3), being the most widely studied...

mRNA in cancer immunotherapy: beyond a source of antigen


Mol Cancer. 2021 Mar 3;20(1):48. doi: 10.1186/s12943-021-01329-3.


mRNA therapeutics have become the focus of molecular medicine research. Various mRNA applications have reached major milestones at high speed in the immuno-oncology field. This can be attributed to the...

Uncertainty analysis of MR-PET image registration for precision neuro-PET imaging


Neuroimage. 2021 May 15;232:117821. doi: 10.1016/j.neuroimage.2021.117821. Epub 2021 Feb 12.


Accurate regional brain quantitative PET measurements, particularly when using partial volume correction, rely on robust image registration between PET and MR images. We argue...

The Next-Generation Immune Checkpoint LAG-3 and Its Therapeutic Potential in Oncology: Third Time's a Charm


Int J Mol Sci. 2020 Dec 23;22(1):75. doi: 10.3390/ijms22010075.


The blockade of immune checkpoints (ICPs), such as cytotoxic T lymphocyte associated protein-4 (CTLA-4) and programmed death-1 (PD-1) and its ligand (PD-L1), has propelled the field of immuno-oncology into...

Neo-Antigen mRNA Vaccines


Vaccines (Basel). 2020 Dec 18;8(4):776. doi: 10.3390/vaccines8040776.


The interest in therapeutic cancer vaccines has caught enormous attention in recent years due to several breakthroughs in cancer research, among which the finding that successful checkpoint blockade...

Targeting Neuropilin-1 with Nanobodies Reduces Colorectal Carcinoma Development


Cancers (Basel). 2020 Nov 30;12(12):3582. doi: 10.3390/cancers12123582.


Neuropilin-1 (NRP-1) is a co-receptor for semaphorins and vascular endothelial growth factor (VEGF) family members that can be expressed on cancer cells and tumor-infiltrating myeloid, endothelial...

Hepatocarcinoma Induces a Tumor Necrosis Factor-Dependent Kupffer Cell Death Pathway That Favors Its Proliferation Upon Partial Hepatectomy


Front Oncol. 2020 Oct 16;10:547013. doi: 10.3389/fonc.2020.547013. eCollection 2020.


Partial hepatectomy (PH) is the main treatment for early-stage hepatocellular carcinoma (HCC). Yet, a significant number of patients undergo recursion of the disease that could be linked...

Anti-human PD-L1 Nanobody for Immuno-PET Imaging: Validation of a Conjugation Strategy for Clinical Translation


Biomolecules. 2020 Sep 29;10(10):1388. doi: 10.3390/biom10101388.


Immune checkpoints, such as programmed death-ligand 1 (PD-L1), limit T-cell function and tumor cells use this ligand to escape the anti-tumor immune response. Treatments with monoclonal antibodies blocking...

Consensus Recommendations on the Use of <sup>18</sup>F-FDG PET/CT in Lung Disease


J Nucl Med. 2020 Dec;61(12):1701-1707. doi: 10.2967/jnumed.120.244780. Epub 2020 Sep 18.


PET with 18 F-FDG has been increasingly applied, predominantly in the research setting, to study drug effects and pulmonary biology and to monitor disease progression and treatment...

Flexible numerical simulation framework for dynamic PET-MR data


Phys Med Biol. 2020 Jul 21;65(14):145003. doi: 10.1088/1361-6560/ab7eee.


This paper presents a simulation framework for dynamic PET-MR. The main focus of this framework is to provide motion-resolved MR and PET data and ground truth motion information. This can be used in...

A randomized controlled phase II clinical trial on mRNA electroporated autologous monocyte-derived dendritic cells (TriMixDC-MEL) as adjuvant treatment for stage III/IV melanoma patients who are disease-free following the resection of macrometastases


Cancer Immunol Immunother. 2020 Dec;69(12):2589-2598. doi: 10.1007/s00262-020-02618-4. Epub 2020 Jun 26.


BACKGROUND: Autologous monocyte-derived mRNA co-electroporated dendritic cells with mRNA encoding CD40 ligand (CD40L), CD70 and a constitutively activated TLR4 (...

Use of non-Gaussian time-of-flight kernels for image reconstruction of Monte Carlo simulated data of ultra-fast PET scanners


EJNMMI Phys. 2020 Jun 19;7(1):42. doi: 10.1186/s40658-020-00309-8.


INTRODUCTION: Time-of-flight (TOF) positron emission tomography (PET) scanners can provide significant benefits by improving the noise properties of reconstructed images. In order to achieve this, the...

Preclinical Targeted α- and β<sup>-</sup>-Radionuclide Therapy in HER2-Positive Brain Metastasis Using Camelid Single-Domain Antibodies


Cancers (Basel). 2020 Apr 21;12(4):1017. doi: 10.3390/cancers12041017.


HER2-targeted therapies have drastically improved the outcome for breast cancer patients. However, when metastasis to the brain is involved, current strategies fail to hold up to the same promise....

TriMix and tumor antigen mRNA electroporated dendritic cell vaccination plus ipilimumab: link between T-cell activation and clinical responses in advanced melanoma


J Immunother Cancer. 2020 Feb;8(1):e000329. doi: 10.1136/jitc-2019-000329.


BACKGROUND: We previously reported that dendritic cell-based mRNA vaccination plus ipilimumab (TriMixDC-MEL IPI) results in an encouraging rate of tumor responses in patients with pretreated...

Effect of attenuation mismatches in time of flight PET reconstruction


Phys Med Biol. 2020 Apr 20;65(8):085009. doi: 10.1088/1361-6560/ab7a6f.


While the pursuit of better time resolution in positron emission tomography (PET) is rapidly evolving, little work has been performed on time of flight (TOF) image quality at high time resolution in...

PET image reconstruction using physical and mathematical modelling for time of flight PET-MR scanners in the STIR library


Methods. 2021 Jan;185:110-119. doi: 10.1016/j.ymeth.2020.01.005. Epub 2020 Jan 30.


This work demonstrates how computational and physical modelling of the positron emission tomography (PET) image acquisition process for a state-of-the-art integrated PET and magnetic...

Correction to: iHIVARNA phase IIa, a randomized, placebo-controlled, double-blinded trial to evaluate the safety and immunogenicity of iHIVARNA-01 in chronically HIV-infected patients under stable combined antiretroviral therapy


Trials. 2019 Dec 13;20(1):721. doi: 10.1186/s13063-019-3940-0.


Following publication of the original article [1], we have been notified that end note would need to be adjusted.

PMID: 31836017 | PMC: PMC6909442 | DOI: 10.1186/s13063-019-3940-0

Therapeutic Vaccine in Chronically HIV-1-Infected Patients: A Randomized, Double-Blind, Placebo-Controlled Phase IIa Trial with HTI-TriMix


Vaccines (Basel). 2019 Dec 6;7(4):209. doi: 10.3390/vaccines7040209.


Therapeutic vaccinations aim to re-educate human immunodeficiency virus (HIV)-1-specific immune responses to achieve durable control of HIV-1 replication in virally suppressed infected individuals after...

PET/MRI attenuation estimation in the lung: A review of past, present, and potential techniques


Med Phys. 2020 Feb;47(2):790-811. doi: 10.1002/mp.13943. Epub 2020 Jan 1.


Positron emission tomography/magnetic resonance imaging (PET/MRI) potentially offers several advantages over positron emission tomography/computed tomography (PET/CT), for example, no CT radiation...

Theranostics in immuno-oncology using nanobody derivatives


Theranostics. 2019 Oct 15;9(25):7772-7791. doi: 10.7150/thno.34941. eCollection 2019.


Targeted therapy and immunotherapy have become mainstream in cancer treatment. However, only patient subsets benefit from these expensive therapies, and often responses are short-lived...

Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use


Biomolecules. 2019 Sep 29;9(10):548. doi: 10.3390/biom9100548.


Immune checkpoint inhibition (ICI) is a promising cancer therapy, which has progressed rapidly from a preclinical concept to clinical implementation. Commonly considered targets in ICI are CTLA-4, PD-1/PD-L1...

Effect of positron range on PET quantification in diseased and normal lungs


Phys Med Biol. 2019 Oct 16;64(20):205010. doi: 10.1088/1361-6560/ab469d.


The impact of positron range on PET image reconstruction has often been investigated as a blurring effect that can be partly corrected by adding an element to the PET system matrix in the...

Commentary: Immunogenic Cell Death and Immunotherapy of Multiple Myeloma


Front Cell Dev Biol. 2019 Jul 31;7:149. doi: 10.3389/fcell.2019.00149. eCollection 2019.


PMID: 31417904 | PMC: PMC6684735 | DOI: 10.3389/fcell.2019.00149

Single Domain Antibody-Mediated Blockade of Programmed Death-Ligand 1 on Dendritic Cells Enhances CD8 T-cell Activation and Cytokine Production


Vaccines (Basel). 2019 Aug 7;7(3):85. doi: 10.3390/vaccines7030085.


Dendritic cell [DC] vaccines can induce durable clinical responses, at least in a fraction of previously treated, late stage cancer patients. Several preclinical studies suggest that shielding programmed...

Intranodal administration of mRNA encoding nucleoprotein provides cross-strain immunity against influenza in mice


J Transl Med. 2019 Jul 25;17(1):242. doi: 10.1186/s12967-019-1991-3.


BACKGROUND: Current human influenza vaccines lack the adaptability to match the mutational rate of the virus and therefore require annual revisions. Because of extensive manufacturing times and the...

Evaluating a Single Domain Antibody Targeting Human PD-L1 as a Nuclear Imaging and Therapeutic Agent


Cancers (Basel). 2019 Jun 21;11(6):872. doi: 10.3390/cancers11060872.


The PD-1:PD-L1 immune checkpoint axis is central in the escape of cancer cells from anticancer immune responses. Monoclonal antibodies (mAbs) specific for PD-L1 have been approved for treatment of...

Perforin and Granzyme B Expressed by Murine Myeloid-Derived Suppressor Cells: A Study on Their Role in Outgrowth of Cancer Cells


Cancers (Basel). 2019 Jun 11;11(6):808. doi: 10.3390/cancers11060808.


A wide-range of myeloid-derived suppressor cell (MDSC)-mediated immune suppressive functions has previously been described. Nevertheless, potential novel mechanisms by which MDSCs aid tumor progression...

iHIVARNA phase IIa, a randomized, placebo-controlled, double-blinded trial to evaluate the safety and immunogenicity of iHIVARNA-01 in chronically HIV-infected patients under stable combined antiretroviral therapy


Trials. 2019 Jun 17;20(1):361. doi: 10.1186/s13063-019-3409-1.


BACKGROUND: HIV therapeutic vaccination aims to improve the immune responses against HIV in order to control viral replication without the need for combined antiretroviral therapy (cART). iHIVARNA-01 is a...

PET Reconstruction With Non-Negativity Constraint in Projection Space: Optimization Through Hypo-Convergence


IEEE Trans Med Imaging. 2020 Jan;39(1):75-86. doi: 10.1109/TMI.2019.2920109. Epub 2019 May 30.


Standard positron emission tomography (PET) reconstruction techniques are based on maximum-likelihood (ML) optimization methods, such as the maximum-likelihood expectation-...

Benefits of Using a Spatially-Variant Penalty Strength With Anatomical Priors in PET Reconstruction


IEEE Trans Med Imaging. 2020 Jan;39(1):11-22. doi: 10.1109/TMI.2019.2913889. Epub 2019 May 23.


In this study, we explore the use of a spatially-variant penalty strength in penalized image reconstruction using anatomical priors to reduce the dependence of lesion contrast...

Towards a personalized iPSC-based vaccine


Nat Biomed Eng. 2018 May;2(5):277-278. doi: 10.1038/s41551-018-0237-7.


PMID: 30936451 | DOI: 10.1038/s41551-018-0237-7

Event-by-event non-rigid data-driven PET respiratory motion correction methods: comparison of principal component analysis and centroid of distribution


Phys Med Biol. 2019 Aug 21;64(16):165014. doi: 10.1088/1361-6560/ab0bc9.


Respiratory motion is a major cause of degradation of PET image quality. Respiratory gating and motion correction can be performed to reduce the effects of respiratory motion; these methods require...

Single-domain antibody fusion proteins can target and shuttle functional proteins into macrophage mannose receptor expressing macrophages


J Control Release. 2019 Apr 10;299:107-120. doi: 10.1016/j.jconrel.2019.02.023. Epub 2019 Feb 21.


The tumor microenvironment of numerous prevalent cancer types is abundantly infiltrated with tumor-associated macrophages (TAMs). Macrophage mannose receptor (MMR or CD206)...

A randomised, placebo-controlled study of omipalisib (PI3K/mTOR) in idiopathic pulmonary fibrosis


Eur Respir J. 2019 Mar 18;53(3):1801992. doi: 10.1183/13993003.01992-2018. Print 2019 Mar.


Phosphatidylinositol 3-kinases (PI3Ks) and mammalian target of rapamycin (mTOR) play a role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Omipalisib (GSK2126458) is a...

Broadening the Message: A Nanovaccine Co-loaded with Messenger RNA and α-GalCer Induces Antitumor Immunity through Conventional and Natural Killer T Cells


ACS Nano. 2019 Feb 26;13(2):1655-1669. doi: 10.1021/acsnano.8b07660. Epub 2019 Feb 15.


Messenger RNA encoding tumor antigens has the potential to evoke effective antitumor immunity. This study reports on a nanoparticle platform, named mRNA Galsomes, that successfully co-...

Immunomodulation of the Tumor Microenvironment: Turn Foe Into Friend


Front Immunol. 2018 Dec 11;9:2909. doi: 10.3389/fimmu.2018.02909. eCollection 2018.


Immunotherapy, where the patient's own immune system is exploited to eliminate tumor cells, has become one of the most prominent new cancer treatment options in the last decade. The main...

Implementation and validation of time-of-flight PET image reconstruction module for listmode and sinogram projection data in the STIR library


Phys Med Biol. 2019 Jan 22;64(3):035004. doi: 10.1088/1361-6560/aaf9b9.


In this paper, we describe the implementation of support for time-of-flight (TOF) positron emission tomography (PET) for both listmode and sinogram data in the open source software for tomographic...

The Vacuolar Pathway of Long Peptide Cross-Presentation Can Be TAP Dependent


J Immunol. 2019 Jan 15;202(2):451-459. doi: 10.4049/jimmunol.1800353. Epub 2018 Dec 17.


The intracellular pathway of cross-presentation, which allows MHC class I-restricted presentation of peptides derived from exogenous Ags, remains poorly defined and may vary with the...

Phase I clinical trial of an intranodally administered mRNA-based therapeutic vaccine against HIV-1 infection


AIDS. 2018 Nov 13;32(17):2533-2545. doi: 10.1097/QAD.0000000000002026.


OBJECTIVE: The efficacy of therapeutic vaccines against HIV-1 infection has been modest. New inerts to redirect responses to vulnerable sites are urgently needed to improve these results.


Epigenetic treatment of multiple myeloma mediates tumor intrinsic and extrinsic immunomodulatory effects


Oncoimmunology. 2018 Jul 23;7(10):e1484981. doi: 10.1080/2162402X.2018.1484981. eCollection 2018.


Immune evasion is an important driver of disease progression in the plasma cell malignancy multiple myeloma. Recent work highlights the potential of epigenetic modulating...

Dendritic Cell Targeting mRNA Lipopolyplexes Combine Strong Antitumor T-Cell Immunity with Improved Inflammatory Safety


ACS Nano. 2018 Oct 23;12(10):9815-9829. doi: 10.1021/acsnano.8b00966. Epub 2018 Oct 1.


In vitro transcribed mRNA constitutes a versatile platform to encode antigens and to evoke CD8 T-cell responses. Systemic delivery of mRNA packaged into cationic liposomes (lipoplexes...

The Journey of <em>in vivo</em> Virus Engineered Dendritic Cells From Bench to Bedside: A Bumpy Road


Front Immunol. 2018 Sep 11;9:2052. doi: 10.3389/fimmu.2018.02052. eCollection 2018.


Dendritic cells (DCs) are recognized as highly potent antigen-presenting cells that are able to stimulate cytotoxic T lymphocyte (CTL) responses with antitumor activity. Consequently, DCs...

Turn Back the TIMe: Targeting Tumor Infiltrating Myeloid Cells to Revert Cancer Progression


Front Immunol. 2018 Aug 31;9:1977. doi: 10.3389/fimmu.2018.01977. eCollection 2018.


Tumor cells frequently produce soluble factors that favor myelopoiesis and recruitment of myeloid cells to the tumor microenvironment (TME). Consequently, the TME of many cancer types is...

Noninvasive imaging of the PD-1:PD-L1 immune checkpoint: Embracing nuclear medicine for the benefit of personalized immunotherapy


Theranostics. 2018 Jun 7;8(13):3559-3570. doi: 10.7150/thno.24762. eCollection 2018.


Molecular imaging of the immune checkpoint receptor PD-1 and its ligand PD-L1 is increasingly investigated as a strategy to guide and monitor PD-1:PD-L1-targeted immune checkpoint...

Non-invasive kinetic modelling of PET tracers with radiometabolites using a constrained simultaneous estimation method: evaluation with <sup>11</sup>C-SB201745


EJNMMI Res. 2018 Jul 3;8(1):58. doi: 10.1186/s13550-018-0412-6.


BACKGROUND: Kinetic analysis of dynamic PET data requires an accurate knowledge of available PET tracer concentration within blood plasma over time, known as the arterial input function (AIF). The gold...

A versatile T cell-based assay to assess therapeutic antigen-specific PD-1-targeted approaches


Oncotarget. 2018 Jun 12;9(45):27797-27808. doi: 10.18632/oncotarget.25591. eCollection 2018 Jun 12.


Blockade of programmed cell death protein 1 (PD-1) immune checkpoint receptor signaling is an established standard treatment for many types of cancer and indications are...

Improved quantitation and reproducibility in multi-PET/CT lung studies by combining CT information


EJNMMI Phys. 2018 Jun 5;5(1):14. doi: 10.1186/s40658-018-0212-0.


BACKGROUND: Matched attenuation maps are vital for obtaining accurate and reproducible kinetic and static parameter estimates from PET data. With increased interest in PET/CT imaging of diffuse lung...

Vegf-A mRNA transfection as a novel approach to improve mouse and human islet graft revascularisation


Diabetologia. 2018 Aug;61(8):1804-1810. doi: 10.1007/s00125-018-4646-7. Epub 2018 May 22.


AIMS/HYPOTHESIS: The initial avascular period following islet transplantation seriously compromises graft function and survival. Enhancing graft revascularisation to improve...

Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation


Oncotarget. 2018 Apr 17;9(29):20476-20489. doi: 10.18632/oncotarget.24944. eCollection 2018 Apr 17.


Lenalidomide is a potent anti-myeloma drug with immunomodulatory properties. It is increasingly used in a low-dose maintenance setting to prolong remission duration after...

Fast Quasi-Newton Algorithms for Penalized Reconstruction in Emission Tomography and Further Improvements via Preconditioning


IEEE Trans Med Imaging. 2018 Apr;37(4):1000-1010. doi: 10.1109/TMI.2017.2786865.


This paper reports on the feasibility of using a quasi-Newton optimization algorithm, limited-memory Broyden-Fletcher-Goldfarb-Shanno with boundary constraints (L-BFGS-B), for penalized...

Clinical Impact of Respiratory Motion Correction in Simultaneous PET/MR, Using a Joint PET/MR Predictive Motion Model


J Nucl Med. 2018 Sep;59(9):1467-1473. doi: 10.2967/jnumed.117.191460. Epub 2018 Mar 9.


In PET imaging, patient motion due to respiration can lead to artifacts and blurring, in addition to quantification errors. The integration of PET imaging with MRI in PET/MRI scanners...

Immune checkpoint blockade combined with IL-6 and TGF-β inhibition improves the therapeutic outcome of mRNA-based immunotherapy


Int J Cancer. 2018 Aug 1;143(3):686-698. doi: 10.1002/ijc.31331. Epub 2018 Mar 13.


Improved understanding of cancer immunology has provided insight into the phenomenon of frequent tumor recurrence after initially successful immunotherapy. A delicate balance exists...

Pulmonary <sup>18</sup>F-FDG uptake helps refine current risk stratification in idiopathic pulmonary fibrosis (IPF)


Eur J Nucl Med Mol Imaging. 2018 May;45(5):806-815. doi: 10.1007/s00259-017-3917-8. Epub 2018 Jan 16.


PURPOSE: There is a lack of prognostic biomarkers in idiopathic pulmonary fibrosis (IPF) patients. The objective of this study is to investigate the potential of 18 F-...

Loss of RASSF4 Expression in Multiple Myeloma Promotes RAS-Driven Malignant Progression


Cancer Res. 2018 Mar 1;78(5):1155-1168. doi: 10.1158/0008-5472.CAN-17-1544. Epub 2017 Dec 19.


RAS mutations occur frequently in multiple myeloma (MM), but apart from driving progression, they can also stimulate antitumor effects by activating tumor-suppressive RASSF...

Issues in quantification of registered respiratory gated PET/CT in the lung


Phys Med Biol. 2017 Dec 14;63(1):015007. doi: 10.1088/1361-6560/aa950b.


PET/CT quantification of lung tissue is limited by several difficulties: the lung density and local volume changes during respiration, the anatomical mismatch between PET and CT and the relative...

Co-delivery of nucleoside-modified mRNA and TLR agonists for cancer immunotherapy: Restoring the immunogenicity of immunosilent mRNA


J Control Release. 2017 Nov 28;266:287-300. doi: 10.1016/j.jconrel.2017.09.041. Epub 2017 Oct 5.


This study reports on the design of mRNA and adjuvant-loaded lipid nanoparticles for therapeutic cancer vaccination. The use of nucleoside-modified mRNA has previously been...

PD1 signal transduction pathways in T cells


Oncotarget. 2017 Apr 19;8(31):51936-51945. doi: 10.18632/oncotarget.17232. eCollection 2017 Aug 1.


The use of immune checkpoint inhibitors for the treatment of cancer is revolutionizing oncology. Amongst these therapeutic agents, antibodies that block PD-L1/PD1...

PDL1 Signals through Conserved Sequence Motifs to Overcome Interferon-Mediated Cytotoxicity


Cell Rep. 2017 Aug 22;20(8):1818-1829. doi: 10.1016/j.celrep.2017.07.075.


PDL1 blockade produces remarkable clinical responses, thought to occur by T cell reactivation through prevention of PDL1-PD1 T cell inhibitory interactions. Here, we find that PDL1 cell-intrinsic...

Antibodies Against Immune Checkpoint Molecules Restore Functions of Tumor-Infiltrating T Cells in Hepatocellular Carcinomas


Gastroenterology. 2017 Oct;153(4):1107-1119.e10. doi: 10.1053/j.gastro.2017.06.017. Epub 2017 Jun 23.


BACKGROUND & AIMS: Ligand binding to inhibitory receptors on immune cells, such as programmed cell death 1 (PD-1) and cytotoxic T-lymphocyte associated protein 4 (...

Non-invasive assessment of murine PD-L1 levels in syngeneic tumor models by nuclear imaging with nanobody tracers


Oncotarget. 2017 Jun 27;8(26):41932-41946. doi: 10.18632/oncotarget.16708.


Blockade of the inhibitory PD-1/PD-L1 immune checkpoint axis is a promising cancer treatment. Nonetheless, a significant number of patients and malignancies do not respond to this therapy. To...

Evaluation of a direct motion estimation/correction method in respiratory-gated PET/MRI with motion-adjusted attenuation


Med Phys. 2017 Jun;44(6):2379-2390. doi: 10.1002/mp.12253. Epub 2017 May 12.


PURPOSE: Respiratory motion compensation in PET/CT and PET/MRI is essential as motion is a source of image degradation (motion blur, attenuation artifacts). In previous work, we developed a...

Sign determination methods for the respiratory signal in data-driven PET gating


Phys Med Biol. 2017 Apr 21;62(8):3204-3220. doi: 10.1088/1361-6560/aa6052. Epub 2017 Mar 27.


Patient respiratory motion during PET image acquisition leads to blurring in the reconstructed images and may cause significant artifacts, resulting in decreased lesion...

Quantification of Lung PET Images: Challenges and Opportunities


J Nucl Med. 2017 Feb;58(2):201-207. doi: 10.2967/jnumed.116.184796. Epub 2017 Jan 12.


Millions of people are affected by respiratory diseases, leading to a significant health burden globally. Because of the current insufficient knowledge of the underlying mechanisms that...

Phosphorylated STAT5 regulates p53 expression via BRCA1/BARD1-NPM1 and MDM2


Cell Death Dis. 2016 Dec 22;7(12):e2560. doi: 10.1038/cddis.2016.430.


Signal transducer and activator of transcription 5 (STAT5) and nucleophosmin (NPM1) are critical regulators of multiple biological and pathological processes. Although a reciprocal regulatory...

Adjuvant-Enhanced mRNA Vaccines


Methods Mol Biol. 2017;1499:179-191. doi: 10.1007/978-1-4939-6481-9_11.


Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA...

Tumour-associated macrophage-mediated survival of myeloma cells through STAT3 activation


J Pathol. 2017 Mar;241(4):534-546. doi: 10.1002/path.4860. Epub 2017 Jan 30.


Overcoming drug resistance is one of the greatest challenges in the treatment of multiple myeloma (MM). The interaction of myeloma cells with the bone marrow (BM) microenvironment is a major...

Combined VEGFR and CTLA-4 blockade increases the antigen-presenting function of intratumoral DCs and reduces the suppressive capacity of intratumoral MDSCs


Am J Cancer Res. 2016 Nov 1;6(11):2514-2531. eCollection 2016.


Melanoma brain metastases (MBM) occur in 10% to 50% of melanoma patients. They are often associated with a high morbidity and despite the improvements in the treatment of advanced melanoma, including...

PETPVC: a toolbox for performing partial volume correction techniques in positron emission tomography


Phys Med Biol. 2016 Nov 21;61(22):7975-7993. doi: 10.1088/0031-9155/61/22/7975. Epub 2016 Oct 25.


Positron emission tomography (PET) images are degraded by a phenomenon known as the partial volume effect (PVE). Approaches have been developed to reduce PVEs, typically...

Preclinical evaluation of an mRNA HIV vaccine combining rationally selected antigenic sequences and adjuvant signals (HTI-TriMix)


AIDS. 2017 Jan 28;31(3):321-332. doi: 10.1097/QAD.0000000000001276.


BACKGROUND: The development of a prophylactic vaccine against HIV-1 has so far not been successful. Therefore, attention has shifted more and more toward the development of novel therapeutic vaccines....

The tumor-associated antigen RHAMM (HMMR/CD168) is expressed by monocyte-derived dendritic cells and presented to T cells


Oncotarget. 2016 Nov 8;7(45):73960-73970. doi: 10.18632/oncotarget.12170.


We formerly demonstrated that vaccination with Wilms' tumor 1 (WT1)-loaded autologous monocyte-derived dendritic cells (mo-DCs) can be a well-tolerated effective treatment in acute myeloid leukemia...

Adult-Derived Human Liver Stem/Progenitor Cells Infused 3 Days Postsurgery Improve Liver Regeneration in a Mouse Model of Extended Hepatectomy


Cell Transplant. 2017 Feb 16;26(2):351-364. doi: 10.3727/096368916X692960. Epub 2016 Sep 21.


There is growing evidence that cell therapy constitutes a promising strategy for liver regenerative medicine. In the setting of hepatic cancer treatments, cell therapy could...

Direct Parametric Reconstruction With Joint Motion Estimation/Correction for Dynamic Brain PET Data


IEEE Trans Med Imaging. 2017 Jan;36(1):203-213. doi: 10.1109/TMI.2016.2594150. Epub 2016 Aug 24.


Direct reconstruction of parametric images from raw photon counts has been shown to improve the quantitative analysis of dynamic positron emission tomography (PET) data....

Lung-resident eosinophils represent a distinct regulatory eosinophil subset


J Clin Invest. 2016 Sep 1;126(9):3279-95. doi: 10.1172/JCI85664. Epub 2016 Aug 22.


Increases in eosinophil numbers are associated with infection and allergic diseases, including asthma, but there is also evidence that eosinophils contribute to homeostatic immune...

Joint PET-MR respiratory motion models for clinical PET motion correction


Phys Med Biol. 2016 Sep 7;61(17):6515-30. doi: 10.1088/0031-9155/61/17/6515. Epub 2016 Aug 15.


Patient motion due to respiration can lead to artefacts and blurring in positron emission tomography (PET) images, in addition to quantification errors. The integration of PET...

Molecular and epigenetic features of melanomas and tumor immune microenvironment linked to durable remission to ipilimumab-based immunotherapy in metastatic patients


J Transl Med. 2016 Aug 2;14(1):232. doi: 10.1186/s12967-016-0990-x.


BACKGROUND: Ipilimumab (Ipi) improves the survival of advanced melanoma patients with an incremental long-term benefit in 10-15 % of patients. A tumor signature that correlates with this survival benefit...

Particle-mediated Intravenous Delivery of Antigen mRNA Results in Strong Antigen-specific T-cell Responses Despite the Induction of Type I Interferon


Mol Ther Nucleic Acids. 2016 Jun 21;5(6):e326. doi: 10.1038/mtna.2016.38.


Cancer vaccines based on mRNA are extensively studied. The fragile nature of mRNA has instigated research into carriers that can protect it from ribonucleases and as such enable its systemic use....

Evaluation of motion-correction methods for dual-gated cardiac positron emission tomography/computed tomography imaging


Nucl Med Commun. 2016 Sep;37(9):956-68. doi: 10.1097/MNM.0000000000000539.


BACKGROUND: Dual gating is a method of dividing the data of a cardiac PET scan into smaller bins according to the respiratory motion and the ECG of the patient. It reduces the undesirable motion...

mRNA Electroporation of Dendritic Cells with WT1, Survivin, and TriMix (a Mixture of caTLR4, CD40L, and CD70)


Methods Mol Biol. 2016;1428:277-83. doi: 10.1007/978-1-4939-3625-0_18.


The immune system is a crucial player in the development of cancer. Once it is in imbalance and immunosuppressive mechanisms supporting tumor growth take over control, dendritic cell immunotherapy...

Key publication Movahedi Group

MMP2 Modulates Inflammatory Response during Axonal Regeneration in the Murine Visual System


Cells. 2021 Jul 2;10(7):1672. doi: 10.3390/cells10071672.


Neuroinflammation has been put forward as a mechanism triggering axonal regrowth in the mammalian central nervous system (CNS), yet little is known about the underlying cellular and molecular players connecting...

CSF1R inhibition rescues tau pathology and neurodegeneration in an A/T/N model with combined AD pathologies, while preserving plaque associated microglia


Acta Neuropathol Commun. 2021 Jun 8;9(1):108. doi: 10.1186/s40478-021-01204-8.


Alzheimer's disease (AD) is characterized by a sequential progression of amyloid plaques (A), neurofibrillary tangles (T) and neurodegeneration (N), constituting ATN pathology. While microglia...

Targeted Repolarization of Tumor-Associated Macrophages via Imidazoquinoline-Linked Nanobodies


Adv Sci (Weinh). 2021 Mar 8;8(10):2004574. doi: 10.1002/advs.202004574. eCollection 2021 May.


Tumor-associated macrophages (TAMs) promote the immune suppressive microenvironment inside tumors and are, therefore, considered as a promising target for the next generation of...

Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization


Nat Neurosci. 2021 Apr;24(4):595-610. doi: 10.1038/s41593-020-00789-y. Epub 2021 Mar 29.


Glioblastomas are aggressive primary brain cancers that recur as therapy-resistant tumors. Myeloid cells control glioblastoma malignancy, but their dynamics during disease...

Therapeutic depletion of CCR8<sup>+</sup> tumor-infiltrating regulatory T cells elicits antitumor immunity and synergizes with anti-PD-1 therapy


J Immunother Cancer. 2021 Feb;9(2):e001749. doi: 10.1136/jitc-2020-001749.


BACKGROUND: Modulation and depletion strategies of regulatory T cells (Tregs) constitute valid approaches in antitumor immunotherapy but suffer from severe adverse effects due to their lack of...

Monocytic myeloid-derived suppressor cells home to tumor-draining lymph nodes via CCR2 and locally modulate the immune response


Cell Immunol. 2021 Apr;362:104296. doi: 10.1016/j.cellimm.2021.104296. Epub 2021 Jan 30.


Efficient priming of anti-tumor T cells requires the uptake and presentation of tumor antigens by immunogenic dendritic cells (DCs) and occurs mainly in lymph nodes draining the...

A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages


Immunity. 2021 Jan 12;54(1):176-190.e7. doi: 10.1016/j.immuni.2020.11.007. Epub 2020 Dec 16.


The developmental and molecular heterogeneity of tissue macrophages is unravelling, as are their diverse contributions to physiology and pathophysiology. Moreover, also given...

Interleukin-10 Prevents Pathological Microglia Hyperactivation following Peripheral Endotoxin Challenge


Immunity. 2020 Nov 17;53(5):1033-1049.e7. doi: 10.1016/j.immuni.2020.09.018. Epub 2020 Oct 12.


Microglia, the resident macrophages of the brain parenchyma, are key players in central nervous system (CNS) development, homeostasis, and disorders. Distinct brain pathologies...

Tightening the retinal glia limitans attenuates neuroinflammation after optic nerve injury


Glia. 2020 Dec;68(12):2643-2660. doi: 10.1002/glia.23875. Epub 2020 Jul 9.


Increasing evidence suggests that functional impairments at the level of the neurovascular unit (NVU) underlie many neurodegenerative and neuroinflammatory diseases. While being part of the NVU,...

Understanding the glioblastoma immune microenvironment as basis for the development of new immunotherapeutic strategies


Elife. 2020 Feb 4;9:e52176. doi: 10.7554/eLife.52176.


Cancer immunotherapy by immune checkpoint blockade has proven its great potential by saving the lives of a proportion of late stage patients with immunogenic tumor types. However, even in these sensitive tumor types,...

Identifying the variables that drive tamoxifen-independent CreERT2 recombination: Implications for microglial fate mapping and gene deletions


Eur J Immunol. 2020 Mar;50(3):459-463. doi: 10.1002/eji.201948162. Epub 2019 Dec 11.


Ligand-dependent Cre recombinases such as the CreERT2 system allow for tamoxifen-inducible Cre recombination. Important examples are the Cx3cr1-CreERT2 and Sall1-CreERT2 lines that are...

Stromal-targeting radioimmunotherapy mitigates the progression of therapy-resistant tumors


J Control Release. 2019 Nov 28;314:1-11. doi: 10.1016/j.jconrel.2019.10.024. Epub 2019 Oct 15.


Radioimmunotherapy (RIT) aims to deliver a high radiation dose to cancer cells, while minimizing the exposure of normal cells. Typically, monoclonal antibodies are used to...

A single-cell atlas of mouse brain macrophages reveals unique transcriptional identities shaped by ontogeny and tissue environment


Nat Neurosci. 2019 Jun;22(6):1021-1035. doi: 10.1038/s41593-019-0393-4. Epub 2019 May 6.


While the roles of parenchymal microglia in brain homeostasis and disease are fairly clear, other brain-resident myeloid cells remain less well understood. By dissecting border...

Single-domain antibody fusion proteins can target and shuttle functional proteins into macrophage mannose receptor expressing macrophages


J Control Release. 2019 Apr 10;299:107-120. doi: 10.1016/j.jconrel.2019.02.023. Epub 2019 Feb 21.


The tumor microenvironment of numerous prevalent cancer types is abundantly infiltrated with tumor-associated macrophages (TAMs). Macrophage mannose receptor (MMR or CD206)...

Clinical Translation of [<sup>68</sup>Ga]Ga-NOTA-anti-MMR-sdAb for PET/CT Imaging of Protumorigenic Macrophages


Mol Imaging Biol. 2019 Oct;21(5):898-906. doi: 10.1007/s11307-018-01302-5.


PURPOSE: Macrophage mannose receptor (MMR, CD206) expressing tumor-associated macrophages (TAM) are protumorigenic and was reported to negatively impact therapy responsiveness and is associated...

Targeting Protumoral Tumor-Associated Macrophages with Nanobody-Functionalized Nanogels through Strain Promoted Azide Alkyne Cycloaddition Ligation


Bioconjug Chem. 2018 Jul 18;29(7):2394-2405. doi: 10.1021/acs.bioconjchem.8b00319. Epub 2018 Jun 28.


Tumor-associated macrophages (TAMs) with high expression levels of the Macrophage Mannose Receptor (MMR, CD206) exhibit a strong angiogenic and immune suppressive...

Endothelial C-Type Natriuretic Peptide Acts on Pericytes to Regulate Microcirculatory Flow and Blood Pressure


Circulation. 2018 Jul 31;138(5):494-508. doi: 10.1161/CIRCULATIONAHA.117.033383.


BACKGROUND: Peripheral vascular resistance has a major impact on arterial blood pressure levels. Endothelial C-type natriuretic peptide (CNP) participates in the local regulation of vascular...

RoMo: An efficient strategy for functional mosaic analysis via stochastic Cre recombination and gene targeting in the ROSA26 locus


Biotechnol Bioeng. 2018 Jul;115(7):1778-1792. doi: 10.1002/bit.26594. Epub 2018 Mar 31.


Functional mosaic analysis allows for the direct comparison of mutant cells with differentially marked control cells in the same organism. While this offers a powerful approach for...

Myeloid cell heterogeneity in cancer: not a single cell alike


Cell Immunol. 2018 Aug;330:188-201. doi: 10.1016/j.cellimm.2018.02.008. Epub 2018 Feb 14.


Tumors of various histological origins show abundant infiltration of myeloid cells from early stages of disease progression. These cells have a profound impact on antitumor immunity...

Beyond the M-CSF receptor - novel therapeutic targets in tumor-associated macrophages


FEBS J. 2018 Feb;285(4):777-787. doi: 10.1111/febs.14202. Epub 2017 Sep 6.


Tumor-associated macrophages (TAM) are by now established as important regulators of tumor progression by impacting on tumor immunity, angiogenesis, and metastasis. Hence, a multitude of...

Novel insights in the regulation and function of macrophages in the tumor microenvironment


Curr Opin Oncol. 2017 Jan;29(1):55-61. doi: 10.1097/CCO.0000000000000344.


PURPOSE OF REVIEW: Tumors contain not only cancer cells but also nontransformed types of cells, the stromal cells. A bidirectional interplay exists between transformed and nontransformed cells...

Odorant receptors can mediate axonal identity and gene choice via cAMP-independent mechanisms


Open Biol. 2016 Jul;6(7):160018. doi: 10.1098/rsob.160018.


Odorant receptors (ORs) control several aspects of cell fate in olfactory sensory neurons (OSNs), including singular gene choice and axonal identity. The mechanisms of OR-induced axon guidance have been suggested...

The Ontogeny and Microenvironmental Regulation of Tumor-Associated Macrophages


Antioxid Redox Signal. 2016 Nov 10;25(14):775-791. doi: 10.1089/ars.2016.6704. Epub 2016 May 17.


SIGNIFICANCE: Tumor progression is supported by non-cancerous stromal cells, of which tumor-associated macrophages (TAMs) are prominent constituents. These cells could be...

Tissue-resident versus monocyte-derived macrophages in the tumor microenvironment


Biochim Biophys Acta. 2016 Jan;1865(1):23-34. doi: 10.1016/j.bbcan.2015.06.009. Epub 2015 Jul 2.


The tumor-promoting role of macrophages has been firmly established in most cancer types. However, macrophage identity has been a matter of debate, since several levels of...

Tumor hypoxia does not drive differentiation of tumor-associated macrophages but rather fine-tunes the M2-like macrophage population


Cancer Res. 2014 Jan 1;74(1):24-30. doi: 10.1158/0008-5472.CAN-13-1196. Epub 2013 Nov 12.


Tumor-associated macrophages (TAM) are exposed to multiple microenvironmental cues in tumors, which collaborate to endow these cells with protumoral activities. Hypoxia, caused by...

Tumor-induced myeloid-derived suppressor cell subsets exert either inhibitory or stimulatory effects on distinct CD8+ T-cell activation events


Eur J Immunol. 2013 Nov;43(11):2930-42. doi: 10.1002/eji.201343349. Epub 2013 Aug 25.


Tumor growth coincides with an accumulation of myeloid-derived suppressor cells (MDSCs), which exert immune suppression and which consist of two main subpopulations, known as monocytic...

Nanobody-based targeting of the macrophage mannose receptor for effective in vivo imaging of tumor-associated macrophages


Cancer Res. 2012 Aug 15;72(16):4165-77. doi: 10.1158/0008-5472.CAN-11-2994. Epub 2012 Jun 19.


Tumor-associated macrophages (TAM) are an important component of the tumor stroma and exert several tumor-promoting activities. Strongly pro-angiogenic TAMs that reside in...

Tumor-associated macrophages in breast cancer: distinct subsets, distinct functions


Int J Dev Biol. 2011;55(7-9):861-7. doi: 10.1387/ijdb.113371dl.


Macrophages display remarkable plasticity, allowing these cells to adapt to changing microenvironments and perform functions as diverse as tissue development and homeostasis, inflammation, pathogen clearance...

Mononuclear phagocyte heterogeneity in cancer: different subsets and activation states reaching out at the tumor site


Immunobiology. 2011 Nov;216(11):1192-202. doi: 10.1016/j.imbio.2011.06.007. Epub 2011 Jun 30.


Mononuclear phagocytes are amongst the most versatile cells of the body, contributing to tissue genesis and homeostasis and safeguarding the balance between pro- and anti-...

Different tumor microenvironments contain functionally distinct subsets of macrophages derived from Ly6C(high) monocytes


Cancer Res. 2010 Jul 15;70(14):5728-39. doi: 10.1158/0008-5472.CAN-09-4672. Epub 2010 Jun 22.


Tumor-associated macrophages (TAM) form a major component of the tumor stroma. However, important concepts such as TAM heterogeneity and the nature of the monocytic TAM...

A method for the isolation and purification of mouse peripheral blood monocytes


J Immunol Methods. 2010 Jul 31;359(1-2):1-10. doi: 10.1016/j.jim.2010.04.004. Epub 2010 May 8.


Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for...

Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes


Blood. 2009 Nov 19;114(21):4664-74. doi: 10.1182/blood-2009-05-221598. Epub 2009 Sep 2.


Alternatively activated macrophages (AAMs), triggered by interleukin-4 (IL-4) and IL-13, play a modulating role during Th2 cytokine-driven pathologies, but their molecular armament...

IL-10 dampens TNF/inducible nitric oxide synthase-producing dendritic cell-mediated pathogenicity during parasitic infection


J Immunol. 2009 Jan 15;182(2):1107-18. doi: 10.4049/jimmunol.182.2.1107.


Antiparasite responses are associated with the recruitment of monocytes that differentiate to macrophages and dendritic cells at the site of infection. Although classically activated monocytic cells...

Macrophages, PPARs, and Cancer


PPAR Res. 2008;2008:169414. doi: 10.1155/2008/169414.


Mononuclear phagocytes often function as control switches of the immune system, securing the balance between pro- and anti-inflammatory reactions. For this purpose and depending on the activating stimuli, these cells...

Identification of discrete tumor-induced myeloid-derived suppressor cell subpopulations with distinct T cell-suppressive activity


Blood. 2008 Apr 15;111(8):4233-44. doi: 10.1182/blood-2007-07-099226. Epub 2008 Feb 13.


The induction of CD11b(+)Gr-1(+) myeloid-derived suppressor cells (MDSCs) is an important immune-evading mechanism used by tumors. However, the exact nature and function of MDSCs...

Classical and alternative activation of mononuclear phagocytes: picking the best of both worlds for tumor promotion


Immunobiology. 2006;211(6-8):487-501. doi: 10.1016/j.imbio.2006.06.002. Epub 2006 Jul 21.


Mononuclear phagocytes often function as control switches of the immune system, securing the balance between pro- and anti-inflammatory reactions. For this purpose and depending on...